IMTEC-t-Transglutaminase Antibodies IgA is a sandwich type enzyme immunoassay. The human recombinant tissue transglutaminase enzyme is attached to microtitration wells. Antigen-specific antibodies in the patient's serum bind to the antigens. Non-specific antibodies are removed by washing. Horse-radish peroxidase labelled goat anti-human IgA are added and bind to human antibodies in the well. The excess conjugate is washed away; then a chromogenic substrate is added. After an appropriate incubation period, the O.D. value is measured by means of a EIA reader (nm 450).

Code ITC30405  96tests